A GING   I NTERVENTION
F OUNDATION

Some representative projects (note: all projects will pass through a comprehensive due diligence process):

Project: Determine how to differentiate adult stem cells into different cell types.
Benefit: Rejuvenate tissues in all the major organs, including heart, lungs, kidneys, liver and pancreas
Organization: L. Stephen Coles MD, PhD and researchers from three major universities
Timeframe: To be determined
Cost: Initial: Set up lab with proper equip to analyze stream of data $250 - 280k
Intermediate: Identify chemical language to activate stem cells. Determine how to identify chemokines that will be tissue specific and turn a pluripotent (can be grown to specialize in any bodily tissue) stem cell into the type of stem cell fated to become an organ. $100-120K
Long term: Develop therapeutic or method. Cost to be determined



Project: Create webcast to present Gerontology Research Group (GRG) meetings worldwide on the web.  Will be digitally stored so they can be viewed at any time.
Benefit: Will allow anyone in the world to “attend” GRG meetings and take advantage of the information presented.
Organization: Gerontology Research Group
Timeframe: Two Weeks
Cost: To be determined



Project: Gather a large amount of biological data from supercentenarians and their relatives, and analyze it to find what they have in common and why they live so long.
Benefit: Allows for precision in the selection of aging therapeutics and methods to develop.
Organization: Supercentenarian Research Foundation
Timeframe: Nine months
Cost: $500 – 520K



Project: A prototype for this project has been completed  Enhance the prototype software system to facilitate information sharing and collaboration among all research organizations conducting projects funded by AIF.
Benefit: Faster project results
Organization: AIF
Timeframe: Two months
Cost: $12k - 15K



Project: Identify, isolate and amplify “pristine” adult stem cells. Currently, adult stem cells in therapeutics or research are replicas of the patients’. That means, a 70 year old patient will be treated with a 70 year olds stem cells. However, we all carry a percentage of stem cells that have escaped damage during our lives or that have had minimal damage or mutations. With a “pristine” stem cell line close to 100% of the stem cells will be in the best condition possible, possibly in the condition the patient enjoyed as a child or teenager. Potential therapies could be to rejuvenate or eventually replace hearts, livers, digestive systems, immune systems, skin, hair and more.
Benefit: Less waste, more effective research and treatment and reduced cancer risk.
Organization: Pristine Stem Cells, Inc. 
Timeframe: 18-24 months
Cost: $2.5 – 3.0M



Project: Understand differences in gene expression that identify declining and toxic cells in order to remove or kill them.
Benefit: Will lead to removal of visceral (around the organs) fat, improved immune response and reduce or eliminate the risk of cancer.
Organization: To be named
Timeframe: 3 – 5 yr.
Cost: Note: Three labs staffed by top scientists are currently pursuing this, and for three separate aging conditions. Projects are currently being funded. AIF will add $5-7M to this effort.



Project: Introduce mitochondrial genes into the cell nucleus -- Thirteen of the approx. 1000 mitochondrial genes are located in the mitochondrion itself, and they are more exposed to damage than mitochondrial genes that are within the cell nucleus. Introducing them into the nucleus would protect them and allow them to continue to work. Three of the 13 have been expressed in the nucleus in cell culture. This project would help get some or all of the rest working in cell culture, the next steps being getting them to work in a mouse, and eventually in humans.
Benefit: Protection from mutations, thereby allowing them to continue to the production of energy, as well as play their parts in a range of other processes (cell signaling, cellular differentiation, apoptosis, control of the cell cycle and cell growth).
Organization: We will fund several of at least a dozen laboratories are working on this problem but with limited and uneven funding.
Timeframe: 5 yr
Cost: AIF would contribute $3-5M to this effort. Note: More than any other project, this would involve collaboration between researchers in labs in locations throughout the world, this project would make good use of the software system to facilitate information sharing and collaboration described in the second item above.



Project: Further refine development of a compound that will break protein crosslinks. Significant work has been done (development of the compound ALT-711 and phase I and II clinical trials) by pharmaceutical companies, but ALT-711 was abandoned because competitors could easily copy. AIF would contribute to the development of others (such as the new compound C36), and possibly extending the ALT-711 clinical trials.
Benefit: Will lead to restoration of elasticity and function of arteries and other parts of the body.
Organization: To be named
Timeframe: 9 mo.
Cost: $1-1.2M initially. We would then evaluate progress and fund accordingly.



Project: A software system created for SRF to manage supercentenarian demographic information. Infrastructure will allow extension for management of biological data. Will allow for data entry and management, and present information on supercentenarians and their relatives.
Benefit: Scientists, researchers and lay people can answer questions, solve problems and make sound decisions.
Organization: AIF in collaboration with Dynabyte Software Co.
Timeframe: Two months
Cost: $10 – 12K

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